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KMID : 0358319960370121345
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Korean Journal of Urology
1996 Volume.37 No. 12 p.1345 ~ p.1350
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Change of MDR Gene Expression and Glutathione Metabolism during Long Stading Low-dose Cisplatin Exposure in Bladder Carcinoma Cell Line
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이은식
김수웅/윤상진/이해원/안한종/이종욱
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Abstract
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Resistance to anticancer chemotherapeutic drugs remains a major obstacle in cancer chemotherapy. A variety of mechanisms rechanisms responsible for drug resistance has been posed. Mdr gene overexpression and detoxification by glutathione are
believed to
be involved in such mechanisms. Recently, we established two low-dose cispatin-resistant human bladder cancer cell lines, T24R0.5 and T24R1, which showed resistance at 0.5 μg/ml and 1 μg/ml of cisplatin, respectively. The resistance of T24R0.5
and
T24R1 cells to cisplatin were 9.4 and 9.37 fold compared to that of the parental T24 cells.
In the study, we investigated the total glutathione content and p-glycoprotein expression, a mdr gene product, in parent and resistant cell lines to elucidate the drug resistance mechanism to cisplatin. Glutathione content was measured by
biochemical
method. P-glycoprotein expression was measured by flowcytometry using monoclonal antibody to p-glycoprotein.
Glutathione content and p-glycoprotein expression not different between parentdal and all resistant cell lines. These results suggest that mdr gene and glutathione do not play a role in cisplatin resistance mechanism in these low-dose
cisplatin-resistant cell lines. Further work will be necessary to determine the mechanism of drug resistance in this model.
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KEYWORD
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